Whole genome amplification in preimplantation genetic diagnosis
نویسندگان
چکیده
منابع مشابه
Title Singleton birth after preimplantation genetic diagnosis for Huntington disease using whole genome amplification
Objective: To report a successful case of preimplantation genetic diagnosis (PGD) for Huntington’s disease using whole genome amplification Design: Case report Setting: University assisted reproduction unit Patient(s): A couple with family history of Huntington’s disease; the husband was carrying the expanded allele of the IT15 gene, while the wife had the normal allele. Intervention(s): PGD wi...
متن کاملSingleton birth after preimplantation genetic diagnosis for Huntington disease using whole genome amplification.
OBJECTIVE To report a successful case of preimplantation genetic diagnosis (PGD) for Huntington disease using whole genome amplification. DESIGN Case report. SETTING University assisted reproduction unit. PATIENT(S) A couple with family history of Huntington disease: The husband was carrying the expanded allele of the IT15 gene, and the wife had the normal allele. INTERVENTION(S) Preimp...
متن کاملPreimplantation diagnosis by whole-genome amplification, PCR amplification, and solid-phase minisequencing of blastomere DNA.
We have developed a new method for preimplantation diagnosis of inherited diseases. Our procedure for the identification of point mutations in single cells combines whole-genome amplification using 15-mer random primers (primer extension preamplification, PEP) with a single locus-specific PCR amplification, followed by detection of the mutation by solid-phase minisequencing. The procedure was e...
متن کاملPreimplantation Genetic Diagnosis
Inherited genetic diseases have been a problem for some families attempting to conceive a child. If affected parents or carriers of genetic disorders wished to avoid transmitting a condition to their child, they can choose to have prenatal diagnosis of their foetus. Amniocentesis or chorionic villus sampling enables cells from the foetus to be collected and sent for genetic analysis. They could...
متن کاملPreimplantation Genetic Diagnosis (PGD)
Preimplantation genetic diagnosis (PGD) is a reproductive technology used with an in vitro fertilization (IVF) cycle to increase the potential for a successful pregnancy and delivery. The most common applications for PGD include testing for extra or missing chromosomes (aneuploidy screening), familial structural chromosome rearrangements, and diagnosis of genetic disease in couples of increased...
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ژورنال
عنوان ژورنال: Journal of Zhejiang University SCIENCE B
سال: 2011
ISSN: 1673-1581,1862-1783
DOI: 10.1631/jzus.b1000196